Non-linear fluorescence excitation of Rhodamine 6G and TRITC labeled IgG in whispering gallery mode microresonators

Carme Pastells; M. Pilar Marco; David Merino-Arranz; Pablo Loza-Alvarez; Laura Pasquardini; Cecilia Pederzolli; Daniele Farnesi; Simone Berneschi; Giancarlo C. Righini; Gualtiero Nunzi Conti; Silvia Soria Huguet

doi:10.1117/12.2079112

3 March 2015 Non-linear fluorescence excitation of Rhodamine 6G and TRITC labeled IgG in whispering gallery mode microresonators

Carme Pastells, M. Pilar Marco, David Merino-Arranz, Pablo Loza-Alvarez, Laura Pasquardini, Cecilia Pederzolli, Daniele Farnesi, Simone Berneschi, Giancarlo C. Righini, Gualtiero Nunzi Conti, Silvia Soria Huguet

Author Affiliations +

Proceedings Volume 9343, Laser Resonators, Microresonators, and Beam Control XVII; 93430K (2015) https://doi.org/10.1117/12.2079112
Event: SPIE LASE, 2015, San Francisco, California, United States

Abstract

We report the non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a microspherical whispering gallery mode resonator (WGMR). The immunoreagents have been immobilized onto the surface of the WGMR sensor after being activated with an epoxy silane and an orienting layer. The developed immunosensor presents great potential as a robust sensing device for fast and early detection of immunoreactions. We also tested the potential of microbubbles as nonlinear enhancement platform. The dyes used in these studies are tetramethyl rhodamine isothiocyanate and Rhodamine 6G. All measurements were performed in a modified confocal microscope.

Citation Download Citation

Carme Pastells, M. Pilar Marco, David Merino-Arranz, Pablo Loza-Alvarez, Laura Pasquardini, Cecilia Pederzolli, Daniele Farnesi, Simone Berneschi, Giancarlo C. Righini, Gualtiero Nunzi Conti, and Silvia Soria Huguet "Non-linear fluorescence excitation of Rhodamine 6G and TRITC labeled IgG in whispering gallery mode microresonators", Proc. SPIE 9343, Laser Resonators, Microresonators, and Beam Control XVII, 93430K (3 March 2015); https://doi.org/10.1117/12.2079112

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available