Imaging highly absorbing nanoparticles using photothermal microscopy

Simon-Alexandre Lussier; Hamid Moradi; Alain Price; Sangeeta Murugkar

doi:10.1117/12.2080278

11 March 2015 Imaging highly absorbing nanoparticles using photothermal microscopy

Simon-Alexandre Lussier, Hamid Moradi, Alain Price, Sangeeta Murugkar

Proceedings Volume 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII; 93370N (2015) https://doi.org/10.1117/12.2080278
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Gold nanoparticles (NPs) have tremendous potential in biomedicine. They can be used as absorbing labels inside living cells for the purpose of biomedical imaging, biosensing as well as for photothermal therapy. We demonstrate photothermal imaging of highly-absorbing particles using a pump-probe setup. The photothermal signal is recovered by heterodyne detection, where the excitation pump laser is at 532 nm and the probe laser is at 638 nm. The sample is moved by a scanning stage. Proof of concept images of red polystyrene microspheres and gold nanoparticles are obtained with this home-built multimodal microscope. The increase in temperature at the surface of the gold NPs, due to the pump laser beam, can be directly measured by means of this photothermal microscope and then compared with the results from theoretical predictions. This technique will be useful for characterization of nanoparticles of different shapes, sizes and materials that are used in cancer diagnosis and therapy.

Citation Download Citation

Simon-Alexandre Lussier, Hamid Moradi, Alain Price, and Sangeeta Murugkar "Imaging highly absorbing nanoparticles using photothermal microscopy", Proc. SPIE 9337, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XII, 93370N (11 March 2015); https://doi.org/10.1117/12.2080278

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