Computational modeling of STED microscopy through multiple biological cells under one- and two-photon excitation

Andrew E. Mark; Mitchell A. Davis; Matthew S. Starosta; Andrew K. Dunn

doi:10.1117/12.2078345

9 March 2015 Computational modeling of STED microscopy through multiple biological cells under one- and two-photon excitation

Andrew E. Mark, Mitchell A. Davis, Matthew S. Starosta, Andrew K. Dunn

Author Affiliations +

Proceedings Volume 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII; 93300V (2015) https://doi.org/10.1117/12.2078345
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

While superresolution optical microscopy techniques afford enhanced resolution for biological applications, they have largely been used to study structures in isolated cells. We use the FDTD method to simulate the propagation of focused beams for STED microscopy through multiple biological cells. We model depletion beams that provide 2D and 3D confinement of the fluorescence spot and assess the effective PSF of the system as a function of focal depth. We compare the relative size of the STED effective PSF under one- and two-photon excitation. PSF calculations suggest that imaging is possible up to the maximum simulation depth if the fluorescence emission remains detectable.

Citation Download Citation

Andrew E. Mark, Mitchell A. Davis, Matthew S. Starosta, and Andrew K. Dunn "Computational modeling of STED microscopy through multiple biological cells under one- and two-photon excitation", Proc. SPIE 9330, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII, 93300V (9 March 2015); https://doi.org/10.1117/12.2078345

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Point spread functions

Stimulated emission depletion microscopy

Electroluminescent displays

Microscopy

Scattering

Optical simulations

Laser scattering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years