Human oral mucosal epithelial cell sheets imaging with high-resolution phase-diversity homodyne OCT

Naoko Senda; Kentaro Osawa

doi:10.1117/12.2076762

2 March 2015 Human oral mucosal epithelial cell sheets imaging with high-resolution phase-diversity homodyne OCT

Naoko Senda, Kentaro Osawa

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93281E (2015) https://doi.org/10.1117/12.2076762
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

There is a need for development of non-invasive technique to evaluate regenerative tissues such as cell sheets for transplantation. We demonstrated non-invasive imaging inside living cell sheets of human oral mucosal epithelial cells by phase-diversity homodyne optical coherence tomography (OCT). The new method OCT developed in Hitachi enables cell imaging because of high resolution (axial resolution; ~2.6 μm, lateral resolution; ~1 μm, in the air). Nuclei inside cell sheets were imaged with sufficient spatial resolution to identify each cell. It suggested that the new method OCT could be useful for non-invasive cell sheet evaluation test.

Citation Download Citation

Naoko Senda and Kentaro Osawa "Human oral mucosal epithelial cell sheets imaging with high-resolution phase-diversity homodyne OCT", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281E (2 March 2015); https://doi.org/10.1117/12.2076762

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KEYWORDS

Optical coherence tomography

Confocal microscopy

Homodyne detection

Image resolution

Microscopes

Tissues

Objectives

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