Microscope-on-chip: combining lens-free microscopy with integrated photonics

Richard Stahl; Dries Vercruysse; Tom Claes; Geert Vanmeerbeeck; Vignesh Mukund; Roelof Jansen; Jeonghwan Song; Luis Hoffman; Xavier Rottenberg; Andy Lambrechts; Liesbet Lagae

doi:10.1117/12.2077484

2 March 2015 Microscope-on-chip: combining lens-free microscopy with integrated photonics

Richard Stahl, Dries Vercruysse, Tom Claes, Geert Vanmeerbeeck, Vignesh Mukund, Roelof Jansen, Jeonghwan Song, Luis Hoffman, Xavier Rottenberg, Andy Lambrechts, Liesbet Lagae

Author Affiliations +

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93281C (2015) https://doi.org/10.1117/12.2077484
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Lens-free in-line Holographic Microscopy (LHM) is a promising imaging technique for many biomedical and industrial applications. The main advantage of the technique is the simplicity of the imaging hardware, requiring no lenses nor high-precision mechanical components. Nevertheless, the LHM systems achieve high imaging performance only in combination with a high-quality and complex illumination. Furthermore, to achieve truly high-throughput imaging capabilities, many applications require a complete on-chip integration. We demonstrate the strength, versatility and scalability of our integrated approach on two microscopes-on-chip instances that combine image sensor technologies with photonics (and micro-fluidics): a fully integrated Point-Source (PS) LHM module for in-flow cell inspection and Large Field-of-View (LFoV) microscope with on-chip photonic illumination for large-area imaging applications. The proposed PS-LHM module consists of a photonic illumination, a micro-fluidic channel and an imager, integrated in a total volume smaller than 0.5 mm³. A low-loss single-mode photonic waveguide is adapted to generate a high- NA illumination spot. Experimental results show strong focusing capabilities and sufficient overall coupling efficiency. Current PS-LHM prototype reaches imaging resolution below 600nm. Our LFoV-LHM system is extremely vertically compact as it consists of only one 1mm-thick illumination chip and one 3mm-thick imaging module. The illumination chip is based on fractal-layout phase-matched waveguides designed to generate multiple light sources that create a quasi-planar illumination wavefront over an area few square millimeter large. Current illumination prototype has active area of approximately 1.2×1.2mm². Our LFoV-LHM prototype reaches imaging resolution of 870nm using image sensor with 1.12μm pixel pitch with maximum FoV of 16.47mm².

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Richard Stahl, Dries Vercruysse, Tom Claes, Geert Vanmeerbeeck, Vignesh Mukund, Roelof Jansen, Jeonghwan Song, Luis Hoffman, Xavier Rottenberg, Andy Lambrechts, and Liesbet Lagae "Microscope-on-chip: combining lens-free microscopy with integrated photonics", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93281C (2 March 2015); https://doi.org/10.1117/12.2077484

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