X-ray phase contrast tomography from whole organ down to single cells

Martin Krenkel; Mareike Töpperwien; Matthias Bartels; Paul Lingor; Detlev Schild; Tim Salditt

doi:10.1117/12.2060390

11 September 2014 X-ray phase contrast tomography from whole organ down to single cells

Martin Krenkel, Mareike Töpperwien, Matthias Bartels, Paul Lingor, Detlev Schild, Tim Salditt

Proceedings Volume 9212, Developments in X-Ray Tomography IX; 92120R (2014) https://doi.org/10.1117/12.2060390
Event: SPIE Optical Engineering + Applications, 2014, San Diego, California, United States

Abstract

We use propagation based hard x-ray phase contrast tomography to explore the three dimensional structure of neuronal tissues from the organ down to sub-cellular level, based on combinations of synchrotron radiation and laboratory sources. To this end a laboratory based microfocus tomography setup has been built in which the geometry was optimized for phase contrast imaging and tomography. By utilizing phase retrieval algorithms, quantitative reconstructions can be obtained that enable automatic renderings without edge artifacts. A high brightness liquid metal microfocus x-ray source in combination with a high resolution detector yielding a resolution down to 1.5 μm. To extend the method to nanoscale resolution we use a divergent x-ray waveguide beam geometry at the synchrotron. Thus, the magnification can be easily tuned by placing the sample at different defocus distances. Due to the small Fresnel numbers in this geometry the measured images are of holographic nature which poses a challenge in phase retrieval.

Citation Download Citation

Martin Krenkel, Mareike Töpperwien, Matthias Bartels, Paul Lingor, Detlev Schild, and Tim Salditt "X-ray phase contrast tomography from whole organ down to single cells", Proc. SPIE 9212, Developments in X-Ray Tomography IX, 92120R (11 September 2014); https://doi.org/10.1117/12.2060390

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