Development and optimization of a line-scanned dual-axis confocal (LS-DAC) microscope for high-speed pathology

Danni Wang; Ye Chen; Daphne Meza; Yu Wang; Jonathan T. C. Liu

doi:10.1117/12.2057743

19 May 2014 Development and optimization of a line-scanned dual-axis confocal (LS-DAC) microscope for high-speed pathology

Danni Wang, Ye Chen, Daphne Meza, Yu Wang, Jonathan T. C. Liu

Author Affiliations +

Proceedings Volume 9155, Translational Biophotonics; 915515 (2014) https://doi.org/10.1117/12.2057743
Event: SPIE Translational Biophotonics, 2014, Houston, Texas, United States

Abstract

We have developed a line-scanned dual-axis confocal (LS-DAC) microscope with subcellular resolution suitable for real time diagnostic imaging at shallow depths. This design serves as a benchtop prototype for a handheld version of the LS-DAC intended for rapid point-of-care pathology. We have assessed the performance trade-offs between the LS-DAC and a point-scanned dual-axis confocal (PS-DAC) microscope via diffraction-theory analysis, Monte-Carlo simulations, and characterization experiments with phantoms and fresh tissues. In addition, we are exploring the use of a sCMOS detector array and rapid 3D deconvolution to improve the sensitivity and resolution of our LS-DAC microscope.

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Danni Wang, Ye Chen, Daphne Meza, Yu Wang, and Jonathan T. C. Liu "Development and optimization of a line-scanned dual-axis confocal (LS-DAC) microscope for high-speed pathology", Proc. SPIE 9155, Translational Biophotonics, 915515 (19 May 2014); https://doi.org/10.1117/12.2057743

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KEYWORDS

Microscopes

Confocal microscopy

Monte Carlo methods

Gradient-index optics

Pathology

Tissues

Tissue optics

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