Spectral analysis of tissues from patients with cancer using a portable spectroscopic diagnostic ratiometer unit

Laura A. Sordillo; Yang Pu; Peter P. Sordillo; Yury Budansky; R. R. Alfano

doi:10.1117/12.2051847

8 May 2014 Spectral analysis of tissues from patients with cancer using a portable spectroscopic diagnostic ratiometer unit

Laura A. Sordillo, Yang Pu, Peter P. Sordillo, Yury Budansky, R. R. Alfano

Author Affiliations +

Proceedings Volume 9129, Biophotonics: Photonic Solutions for Better Health Care IV; 91292R (2014) https://doi.org/10.1117/12.2051847
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

Spectral profiles of tissues from patients with breast carcinoma, malignant carcinoid and non-small cell lung carcinoma were acquired using native fluorescence spectroscopy. A novel spectroscopic ratiometer device (S³-LED) with selective excitation wavelengths at 280 nm and 335 nm was used to produce the emission spectra of the key biomolecules, tryptophan and NADH, in the tissue samples. In each of the samples, analysis of emission intensity peaks from biomolecules showed increased 340 nm/440 nm and 340 nm/460 nm ratios in the malignant samples compared to their paired normal samples. This most likely represented increased tryptophan to NADH ratios in the malignant tissue samples compared to their paired normal samples. Among the non-small cell lung carcinoma and breast carcinomas, it appeared that tumors of very large size or poor differentiation had an even greater increase in the 340 nm/440 nm and 340 nm/460 nm ratios. In the samples of malignant carcinoid, which is known to be a highly metabolically active tumor, a marked increase in these ratios was also seen.

Citation Download Citation

Laura A. Sordillo, Yang Pu, Peter P. Sordillo, Yury Budansky, and R. R. Alfano "Spectral analysis of tissues from patients with cancer using a portable spectroscopic diagnostic ratiometer unit", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91292R (8 May 2014); https://doi.org/10.1117/12.2051847

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