Fluorescence Studies Of Bilayers And Proteins: Critical Behavior And Genetic Engineering

Bruce Hudson; Anthony Ruggiero; Danni Harris; lain Johnson; Xiu -mei Dou; Thomas Novet; Lawrence McIntosh; Cynthia Phillips; Theresa Nester

doi:10.1117/12.945375

24 June 1988 Fluorescence Studies Of Bilayers And Proteins: Critical Behavior And Genetic Engineering

Bruce Hudson, Anthony Ruggiero, Danni Harris, lain Johnson, Xiu -mei Dou, Thomas Novet, Lawrence McIntosh, Cynthia Phillips, Theresa Nester

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945375
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Fluorescence decay and anisotropy studies of parinaric acid in phospholipid bilayers have demonstrated the presence of density fluctuations exhibiting critical behavior in these nominally single phase structures. The anisotropy behavior is used to extract the second and fourth rank order parameters <P2> and <P4> for comparison with models of acyl chain order. A recent study of parinaric acid in hexagonal urea inclusion complexes has demonstrated that the strongly allowed transition is polarized at an angle with respect to the chain axis. The implications of this for the interpretation of anisotropy experiments is discussed. The lysozyme from bacteriophage aq has been engineered in modified forms containing only one tryptophan residue and with substitutions near the buried tryptophan residue 138. Simple changes in the structure appear to result in large changes in the dynamics of this residue. These observations are compared with the results of molecular dynamics computations.

Citation Download Citation

Bruce Hudson, Anthony Ruggiero, Danni Harris, lain Johnson, Xiu -mei Dou, Thomas Novet, Lawrence McIntosh, Cynthia Phillips, and Theresa Nester "Fluorescence Studies Of Bilayers And Proteins: Critical Behavior And Genetic Engineering", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945375

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available