Progress report towards a digital mirror device based confocal microscopic system

Dingrong Yi; Shunhua Lin; Simian Huang; Shaochuan Xie

doi:10.1117/12.2041746

19 December 2013 Progress report towards a digital mirror device based confocal microscopic system

Dingrong Yi, Shunhua Lin, Simian Huang, Shaochuan Xie

Proceedings Volume 9046, 2013 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems; 90460K (2013) https://doi.org/10.1117/12.2041746
Event: International Conference on Optical Instruments and Technology (OIT2013), 2013, Beijing, China

Abstract

It is widely believed that by using a digital mirror device (DMD) as the spatial light modulator (SLM) of a programmable array microcopy (PAM), it is possible to achieve a cost-effective alternative to expensive confocal imaging devices. During the past decade, the design of such a DMD based PAM instrument has been frequently reported to enhance resolution and contrast, convincing images with improved quality are rare to be seen. The concrete implementation of a DMD based PAM instrument needs to successfully resolve multiple issues such as the adverse effects caused by the tilt angle of the micro-mirrors from the base board, the registration between a micro mirror of the DMD and the image pixel of the photo-detector and so on. In this paper, we report the design of a middle body consisting of a DMD as an independent attachment to a conventional microscope to convert the latter into a confocal imaging system, in a similar way as a filter turret that is placed below the head and the objectives of a regular microscopy to convert it into a fluorescent microscopy. Images of real objects with improved contrast are provided to demonstrate the effectiveness of using a DMD as SLM to improve the contrast of a PAM instrument. Such a PAM instrument has many advantages compared to conventional laser-scanning confocal systems including lower costs and higher imaging speeds. In addition, it allows convenient dynamic adjustments between imaging quality and imaging speed.

Citation Download Citation

Dingrong Yi, Shunhua Lin, Simian Huang, and Shaochuan Xie "Progress report towards a digital mirror device based confocal microscopic system", Proc. SPIE 9046, 2013 International Conference on Optical Instruments and Technology: Optoelectronic Measurement Technology and Systems, 90460K (19 December 2013); https://doi.org/10.1117/12.2041746

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