Intramyocellular lipid dependence on skeletal muscle fiber type and orientation characterized by diffusion tensor imaging and 1H-MRS

Sunil K. Valaparla; Feng Gao; Muhammad Abdul-Ghani; Geoffrey D. Clarke

doi:10.1117/12.2041728

21 March 2014 Intramyocellular lipid dependence on skeletal muscle fiber type and orientation characterized by diffusion tensor imaging and ¹H-MRS

Sunil K. Valaparla, Feng Gao, Muhammad Abdul-Ghani, Geoffrey D. Clarke

Author Affiliations +

Proceedings Volume 9034, Medical Imaging 2014: Image Processing; 90340S (2014) https://doi.org/10.1117/12.2041728
Event: SPIE Medical Imaging, 2014, San Diego, California, United States

Abstract

When muscle fibers are aligned with the B₀ field, intramyocellular lipids (IMCL), important for providing energy during physical activity, can be resolved in proton magnetic resonance spectra (¹H-MRS). Various muscles of the leg differ significantly in their proportion of fibers and angular distribution. This study determined the influence of muscle fiber type and orientation on IMCL using ¹H-MRS and diffusion tensor imaging (DTI). Muscle fiber orientation relative to B₀ was estimated by pennation angle (PA) measurements from DTI, providing orientation-specific extramyocellular lipid (EMCL) chemical shift data that were used for subject-specific IMCL quantification. Vastus lateralis (VL), tibialis anterior (TA) and soleus (SO) muscles of 6 healthy subjects (21-40 yrs) were studied on a Siemens 3T MRI system with a flex 4-channel coil. ¹H-MRS were acquired using stimulated echo acquisition mode (STEAM, TR=3s, TE=270ms). DTI was performed using single shot EPI (b=600s/mm², 30 directions, TR=4.5s, TE=82ms, and ten×5mm slices) with center slice indexed to the MRS voxel. The average PA’s measured from ROI analysis of primary eigenvectors were PA=19.46±5.43 for unipennate VL, 15.65±3.73 for multipennate SO, and 7.04±3.34 for bipennate TA. Chemical shift (CS) was calculated using [3cos²θ-1] dependence: 0.17±0.02 for VL, 0.18±0.01 for SO and 0.19±0.004 ppm for TA. IMCL-CH₂ concentrations from spectral analysis were 12.77±6.3 for VL, 3.07±1.63 for SO and 0.27±0.08 mmol/kg ww for TA. Small PA’s were measured in TA and large CS with clear separation between EMCL and IMCL peaks were observed. Larger variations in PA were measured VL and SO resulting in an increased overlap of the EMCL on IMCL peaks.

Citation Download Citation

Sunil K. Valaparla, Feng Gao, Muhammad Abdul-Ghani, and Geoffrey D. Clarke "Intramyocellular lipid dependence on skeletal muscle fiber type and orientation characterized by diffusion tensor imaging and ¹H-MRS", Proc. SPIE 9034, Medical Imaging 2014: Image Processing, 90340S (21 March 2014); https://doi.org/10.1117/12.2041728

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Diffusion tensor imaging

Chemical analysis

Diffusion

Magnetic resonance imaging

Magnetism

Imaging spectroscopy

Scanners

Show All Keywords

Keywords/Phrases

Search In:

Publication Years