Immune biosensors based on the SPR and TIRE: efficiency of their application for bacteria determination

N. F. Starodub; J. Ogorodniichuk; T. Lebedeva; P. Shpylovyy

doi:10.1117/12.2044648

18 November 2013 Immune biosensors based on the SPR and TIRE: efficiency of their application for bacteria determination

N. F. Starodub, J. Ogorodniichuk, T. Lebedeva, P. Shpylovyy

Author Affiliations +

Proceedings Volume 9032, Biophotonics—Riga 2013; 90320X (2013) https://doi.org/10.1117/12.2044648
Event: 1st International Conference "Biophotonics Riga 2013", 2013, Riga, Latvia

Abstract

In this work we have designed high-specific biosensors for Salmonella typhimurium detection based on the surface plasmon resonance (SPR) and total internal reflection ellipsometry (TIRE). It has been demonstrated high selectivity and sensitivity of analysis. As a registering part for our experiments the Spreeta (USA) and “Plasmonotest” (Ukraine) with flowing cell have been applied among of SPR device. Previous researches confirmed an efficiency of SPR biosensors using for detecting of specific antigen-antibody interactions therefore this type of reactions with some previous preparations of surface binding layer was used as reactive part. It has been defined that in case with Spreeta sensitivity was on the level 10³ – 10⁷ cells/ml. Another biosensor based on the SPR has shown the sensitivity within 10¹ – 10⁶ cells/ml. Maximal sensitivity was on the level of several cells in 10 ml (up to the fact that less than 5 cells) which has been obtained using the biosensor based on TIRE.

Citation Download Citation

N. F. Starodub, J. Ogorodniichuk, T. Lebedeva, and P. Shpylovyy "Immune biosensors based on the SPR and TIRE: efficiency of their application for bacteria determination", Proc. SPIE 9032, Biophotonics—Riga 2013, 90320X (18 November 2013); https://doi.org/10.1117/12.2044648

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available