Disposable pen-shaped capillary gel electrophoresis cartridge for fluorescence detection of bio-molecules

Varoujan Amirkhanian; Shou-Kuan Tsai

doi:10.1117/12.2034036

6 March 2014 Disposable pen-shaped capillary gel electrophoresis cartridge for fluorescence detection of bio-molecules

Varoujan Amirkhanian, Shou-Kuan Tsai

Author Affiliations +

Proceedings Volume 8976, Microfluidics, BioMEMS, and Medical Microsystems XII; 897610 (2014) https://doi.org/10.1117/12.2034036
Event: SPIE MOEMS-MEMS, 2014, San Francisco, California, United States

Abstract

We introduce a novel and cost-effective capillary gel electrophoresis (CGE) system utilizing disposable pen-shaped gelcartridges for highly efficient, high speed, high throughput fluorescence detection of bio-molecules. The CGE system has been integrated with dual excitation and emission optical-fibers with micro-ball end design for fluorescence detection of bio-molecules separated and detected in a disposable pen-shaped capillary gel electrophoresis cartridge. The high-performance capillary gel electrophoresis (CGE) analyzer has been optimized for glycoprotein analysis type applications. Using commercially available labeling agent such as ANTS (8-aminonapthalene-1,3,6- trisulfonate) as an indicator, the capillary gel electrophoresis-based glycan analyzer provides high detection sensitivity and high resolving power in 2-5 minutes of separations. The system can hold total of 96 samples, which can be automatically analyzed within 4-5 hours. This affordable fiber optic based fluorescence detection system provides fast run times (4 minutes vs. 20 minutes with other CE systems), provides improved peak resolution, good linear dynamic range and reproducible migration times, that can be used in laboratories for high speed glycan (N-glycan) profiling applications. The CGE-based glycan analyzer will significantly increase the pace at which glycoprotein research is performed in the labs, saving hours of preparation time and assuring accurate, consistent and economical results.

Citation Download Citation

Varoujan Amirkhanian and Shou-Kuan Tsai "Disposable pen-shaped capillary gel electrophoresis cartridge for fluorescence detection of bio-molecules", Proc. SPIE 8976, Microfluidics, BioMEMS, and Medical Microsystems XII, 897610 (6 March 2014); https://doi.org/10.1117/12.2034036

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE