A non-invasive diffuse reflectance calibration-free method for absolute determination of exogenous biochemicals concentration in biological tissues

Alexander V. Lappa; Artem N. Kulikovskiy; Oleg G. Busarov

doi:10.1117/12.2036337

4 March 2014 A non-invasive diffuse reflectance calibration-free method for absolute determination of exogenous biochemicals concentration in biological tissues

Alexander V. Lappa, Artem N. Kulikovskiy, Oleg G. Busarov

Author Affiliations +

Proceedings Volume 8952, Biomedical Applications of Light Scattering VIII; 895216 (2014) https://doi.org/10.1117/12.2036337
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The paper presents a new method for distant non-destructive determination of concentration of light absorbing admixtures in turbid media. In particular, it is intended for non-invasive in vivo control of accumulation in patient tissues of various biochemicals introduced to the patients for chemotherapy, photodynamic therapy or diagnostics. It is require that the admixture absorption spectrum should have a clearly marked peak in the wavelength region where the pure medium one varies regularly. Fluorescence of admixtures is not required. The method uses the local diffuse reflectance spectroscopy with optical fiber probe including one emitting and two reading There are several features in the method: the value to be determined is absolute concentration of admixtures; the method needs no calibration measurements on phantoms; it needs no reference measurements on sample with zero admixture concentration; it uses a two parametric kinetic light propagation model and original algorithms to resolve direct and inverse tasks of radiation transport theory. Experimental testing passed with tissue equivalent phantoms and different admixtures, including a chlorine photosensitizer, showed accuracy under 10% in all cases.

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Alexander V. Lappa, Artem N. Kulikovskiy, and Oleg G. Busarov "A non-invasive diffuse reflectance calibration-free method for absolute determination of exogenous biochemicals concentration in biological tissues", Proc. SPIE 8952, Biomedical Applications of Light Scattering VIII, 895216 (4 March 2014); https://doi.org/10.1117/12.2036337

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KEYWORDS

Absorption

Sensors

Fabry–Perot interferometers

Optical fibers

Spectroscopy

Tissues

Diffuse reflectance spectroscopy

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