Rate-dependent dynamics of cellular membranes probed by laser tweezers and optical displacement sensing

Nima Khatibzadeh; Alexander A. Spector; William E. Brownell; Bahman Anvari

doi:10.1117/12.2039835

26 February 2014 Rate-dependent dynamics of cellular membranes probed by laser tweezers and optical displacement sensing

Nima Khatibzadeh, Alexander A. Spector, William E. Brownell, Bahman Anvari

Author Affiliations +

Proceedings Volume 8946, Optical Elastography and Tissue Biomechanics; 89460P (2014) https://doi.org/10.1117/12.2039835
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

In this study, we investigated nanomechanical properties of cell membranes in response to elongation at different rates. Membrane nanotubes (tethers) were pulled at different pulling rates by an optically-trapped fluorescent microsphere as recorded and analyzed for low (1 μm/s) and high (100 μm/s) pulling rates. The force relaxation response of membrane nanotubes exhibited a bi-phasic behavior including fast and slow relaxation processes at low and high pulling rates. The fast and slow force relaxation time constants were 0.388±0.21 s and 11.74±3.35 s, in response to pulling rate of 1 μm/s, respectively and significantly decreased at higher pulling rates. These reductions in the time constants are suggestive of reduced viscous effects and weakened adhesions between the membrane and the cytoskeleton during rapid pulling.

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Nima Khatibzadeh, Alexander A. Spector, William E. Brownell, and Bahman Anvari "Rate-dependent dynamics of cellular membranes probed by laser tweezers and optical displacement sensing", Proc. SPIE 8946, Optical Elastography and Tissue Biomechanics, 89460P (26 February 2014); https://doi.org/10.1117/12.2039835

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