Linking cell shape, elasticity and fate: in vitro re-differentiation of chondrocytes

Xiaofei Yuan; Yahua Chim; Huabing Yin

doi:10.1117/12.2039859

26 February 2014 Linking cell shape, elasticity and fate: in vitro re-differentiation of chondrocytes

Xiaofei Yuan, Yahua Chim, Huabing Yin

Proceedings Volume 8946, Optical Elastography and Tissue Biomechanics; 89460M (2014) https://doi.org/10.1117/12.2039859
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Autologous chondrocyte transplantation (ACT) has become a promising method for repairing large articular defects. However, dedifferentiation of chondrocytes during cell expansion remains a major limitation for ACT procedures. In this study, we explore the potential of confining cell shape for re-differentiation of dedifferentiated bovine chondrocytes. A novel culture system, combining 2D micropatterning with 3D matrix formation, was developed to control and maintain individual chondrocyte’s shape. Both collagen II synthesis and the mechanical properties of cells were monitored during re-differentiation. We show that a spherical morphology without cell spreading plays a limited role in induction of re-differentiation. Instead, isolated, dedifferentiated chondrocytes partially regain chondrogenic properties if they have an appropriate cell shape and limited spreading.

Citation Download Citation

Xiaofei Yuan, Yahua Chim, and Huabing Yin "Linking cell shape, elasticity and fate: in vitro re-differentiation of chondrocytes", Proc. SPIE 8946, Optical Elastography and Tissue Biomechanics, 89460M (26 February 2014); https://doi.org/10.1117/12.2039859

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