Photothermal bleaching and recovery analysis in photoacoustic microscopy

Chiye Li; Chi Zhang; Liang Gao; Alejandro Garcia-Uribe; Lihong V. Wang

doi:10.1117/12.2040936

3 March 2014 Photothermal bleaching and recovery analysis in photoacoustic microscopy

Chiye Li, Chi Zhang, Liang Gao, Alejandro Garcia-Uribe, Lihong V. Wang

Proceedings Volume 8943, Photons Plus Ultrasound: Imaging and Sensing 2014; 89435Q (2014) https://doi.org/10.1117/12.2040936
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

A novel method – photoacoustic recovery after photothermal bleaching (PRAP) – is proposed and implemented to study particle dynamics and medium properties at the micron scale via photoacoustic imaging. PRAP is an intuitive way to visualize as well as quantify dynamic processes in many kinds of media. We demonstrate PRAP first in a phantom study, and then in live cells. PRAP provides high signal-to-noise ratio imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic phenomena inside a cell.

Citation Download Citation

Chiye Li, Chi Zhang, Liang Gao, Alejandro Garcia-Uribe, and Lihong V. Wang "Photothermal bleaching and recovery analysis in photoacoustic microscopy", Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89435Q (3 March 2014); https://doi.org/10.1117/12.2040936

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available