A Spectral Method For The Rapid Detection And Identification Of Human Viruses

Alan Pronovost; Pei Chang; Wilfred H. Nelson

doi:10.1117/12.955028

20 January 1977 A Spectral Method For The Rapid Detection And Identification Of Human Viruses

Alan Pronovost, Pei Chang, Wilfred H. Nelson

Proceedings Volume 0089, Applications of Optics in Medicine and Biology; (1977) https://doi.org/10.1117/12.955028
Event: 20th Annual Technical Symposium, 1976, San Diego, United States

Abstract

Poliovirus type I, strain LSc2ab, has been grown in HeLa cell cultures and purified by means of a five-step process involving cell debris removal, virus precipitation with polyethylene glycol 6000, ultrasedimentation into a discontinuous CsCl step gradient, chloroform extraction in the hypertonic state, and isopycnic density gradient centrifugation. Virus concentration of 1.5-3.0 mg/ml in 20 μliter samples was achieved by volume reduction. Laser-Raman spectra of the purified samples exhibit characteristic lines which can be assigned to the viral coat protein and the RNA. Because characteristic spectral "fingerprints" can be obtained in a matter of minutes, it may be possible to develop a method of rapid viral analysis based upon molecular spectroscopy. Our study shows for the first time that a human virus can be identified by Raman spectroscopy.

Citation Download Citation

Alan Pronovost, Pei Chang, and Wilfred H. Nelson "A Spectral Method For The Rapid Detection And Identification Of Human Viruses", Proc. SPIE 0089, Applications of Optics in Medicine and Biology, (20 January 1977); https://doi.org/10.1117/12.955028

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