Detection of saccharides with a fluorescent sensing device based on a  gold film modified with 
4-mercaptophenylboronic acid monolayer

Shu-Jen Chen; Jui-Feng Chang; Nai-Jen Cheng; Jeng-Nan Yih; Kuo-Chi Chiu

doi:10.1117/12.2023060

11 September 2013 Detection of saccharides with a fluorescent sensing device based on a gold film modified with 4-mercaptophenylboronic acid monolayer

Shu-Jen Chen, Jui-Feng Chang, Nai-Jen Cheng, Jeng-Nan Yih, Kuo-Chi Chiu

Author Affiliations +

Proceedings Volume 8812, Biosensing and Nanomedicine VI; 881210 (2013) https://doi.org/10.1117/12.2023060
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

An extremely sensitive fluorescent sensor based on a phenylboronic acid monolayer was developed for detecting saccharide molecules. The fluorescent sensor was prepared by assembling a monolayer of 4-mercaptophenylboronic acid (4-MPBA) onto a gold-coated compact disk. The change in the fluorescence of the 4-MPBA monolayer was extremely obvious in basic methanolic buffer containing monosaccharides down to the picomolar level. The fluorescence spectra demonstrated that the 4-MPBA monolayer was sensitive to monosaccharides and disaccharides, and the affinity of the monolayer toward saccharides was in the order of glucose < fructose < mannose < galactose < maltose > lactose > sucrose. Additionally, the fluorescence intensity of 4-MPBA monolayer was restorable after cleaning with weak acid, indicating that the reported fluorescent sensor with the detection limit of glucose down to the picomolar level is reusable for sensing saccharides.

Citation Download Citation

Shu-Jen Chen, Jui-Feng Chang, Nai-Jen Cheng, Jeng-Nan Yih, and Kuo-Chi Chiu "Detection of saccharides with a fluorescent sensing device based on a gold film modified with 4-mercaptophenylboronic acid monolayer", Proc. SPIE 8812, Biosensing and Nanomedicine VI, 881210 (11 September 2013); https://doi.org/10.1117/12.2023060

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