Probing cell activity in random access modality

L. Sacconi; C. Crocini; J. Lotti; R. Coppini; C. Ferrantini; C. Tesi; P. Yan; L. M. Loew; E. Cerbai; C. Poggesi; F. S. Pavone

doi:10.1117/12.2030585

24 June 2013 Probing cell activity in random access modality

L. Sacconi, C. Crocini, J. Lotti, R. Coppini, C. Ferrantini, C. Tesi, P. Yan, L. M. Loew, E. Cerbai, C. Poggesi, F. S. Pavone

Author Affiliations +

Proceedings Volume 8804, Neurophotonics; 880406 (2013) https://doi.org/10.1117/12.2030585
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

We combined the advantage of an ultrafast random access microscope with novel labelling technologies to study the intra- and inter-cellular action potential propagation in neurons and cardiac myocytes with sub-millisecond time resolution. The random accesses microscopy was used in combination with a new fluorinated voltage sensitive dye with improved photostability to record membrane potential from multiple Purkinje cells with near simultaneous sampling. The RAMP system rapidly scanned between lines drawn in the membranes of neurons to perform multiplex measurements of the TPF signal. This recording was achieved by rapidly positioning the laser excitation with the AOD to sample a patch of membrane from each cell in <100 μs; for recording from five cells, multiplexing permits a temporal resolution of 400 μs sufficient to capture every spike. The system is capable to record spontaneous activity over 800 ms from five neighbouring cells simultaneously, showing that spiking is not temporally correlated. The system was also used to investigate the electrical properties of tubular system (TATS) in isolated rat ventricular myocytes.

Citation Download Citation

L. Sacconi, C. Crocini, J. Lotti, R. Coppini, C. Ferrantini, C. Tesi, P. Yan, L. M. Loew, E. Cerbai, C. Poggesi, and F. S. Pavone "Probing cell activity in random access modality", Proc. SPIE 8804, Neurophotonics, 880406 (24 June 2013); https://doi.org/10.1117/12.2030585

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available