Infrared imaging of fixed-cells through micro-cavity fiber optic scanning microscopy

A. Di Donato; T. Pietrangelo; T. Da Ros; T. Monti; D. Mencarelli; G. Venanzoni; A. Morini; M. Farina

doi:10.1117/12.2032464

17 June 2013 Infrared imaging of fixed-cells through micro-cavity fiber optic scanning microscopy

A. Di Donato, T. Pietrangelo, T. Da Ros, T. Monti, D. Mencarelli, G. Venanzoni, A. Morini, M. Farina

Proceedings Volume 8797, Advanced Microscopy Techniques III; 87970I (2013) https://doi.org/10.1117/12.2032464
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

Noninvasive, lens-free microscopy methods helps biologists to measure quantitative contrast phase imaging without damaging the cells. An extrinsic scanning micro-cavity in optical fiber is proposed to achieve surface imaging at infrared wavelengths. The micro-cavity is realized by approaching a single mode fiber with a numerical aperture NA to a sample and it is fed by a low-coherence source. The measurement of the reflected optical intensity provides a map of the sample reflectivity, whereas from the analysis of the reflected spectrum in the time/spatial domain, we disentangle the topography and contrast phase information. The latter describes the contrast variation of the reflected spectrum from the cavity due to changes in topography and surface refractive index. The interference of diffracted waves defines the transverse field behavior of the electromagnetic field inside the micro-cavity, affecting in this way the transverse resolution, that is not defined by the numerical aperture NA of the fiber and consequently by the conventional Rayleigh limit (about 0.6λ/NA). The resolution in the normal direction is limited mainly by the source bandwidth and demodulation algorithm. The system shows a compact and simple architecture.

Citation Download Citation

A. Di Donato, T. Pietrangelo, T. Da Ros, T. Monti, D. Mencarelli, G. Venanzoni, A. Morini, and M. Farina "Infrared imaging of fixed-cells through micro-cavity fiber optic scanning microscopy", Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970I (17 June 2013); https://doi.org/10.1117/12.2032464

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available