Deconvolution microscopy of living cells for phase-contrast imaging

Guanxiao Cheng; Ping Xu; Zhilong Sun; Chunquan Hong; Zelin Li

doi:10.1117/12.2020372

13 May 2013 Deconvolution microscopy of living cells for phase-contrast imaging

Guanxiao Cheng, Ping Xu, Zhilong Sun, Chunquan Hong, Zelin Li

Proceedings Volume 8789, Modeling Aspects in Optical Metrology IV; 87890F (2013) https://doi.org/10.1117/12.2020372
Event: SPIE Optical Metrology 2013, 2013, Munich, Germany

Abstract

The goal of deconvolution microscopy for phase-contrast imaging is to reassign the optical blur to its original position and to reduce statistical noise, thus visualizing the cellular structures of living cells in three dimensions and at subresolution scale. The major features of this technology for a phase-contrast microscopy are discussed through a series of theoretical analyses. A few of possible sources of aberrations and image degradation processes are presented. The theoretical and experimental results have shown that deconvolution microscopy can enhance resolution and contrast by either subtracting or reassigning out-of-focus blur.

Citation Download Citation

Guanxiao Cheng, Ping Xu, Zhilong Sun, Chunquan Hong, and Zelin Li "Deconvolution microscopy of living cells for phase-contrast imaging", Proc. SPIE 8789, Modeling Aspects in Optical Metrology IV, 87890F (13 May 2013); https://doi.org/10.1117/12.2020372

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