Single molecule interactions studied by using a modified DNA sequencer: a comparison with surface plasmon resonance

Jens Sobek; Stefan Schauer; Hubert Rehrauer; David Fischer; Andrea Patrignani; Stephan Landgraf; Ralph Schlapbach

doi:10.1117/12.2002953

21 February 2013 Single molecule interactions studied by using a modified DNA sequencer: a comparison with surface plasmon resonance

Jens Sobek, Stefan Schauer, Hubert Rehrauer, David Fischer, Andrea Patrignani, Stephan Landgraf, Ralph Schlapbach

Author Affiliations +

Proceedings Volume 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V; 85960Y (2013) https://doi.org/10.1117/12.2002953
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In this study we established the principles for using a commercial off-the-shelf DNA sequencer (RS, Pacific Biosciences) to measure biomolecular interactions. Binding between single oligonucleotides immobilized at the bottom of a nanowell to a complementary DNA strand was used as a model system. The influence of the labeling dye on the hybridization of a 7mer oligonucleotide was investigated. The resulting association and dissociation rate constants (k_on and k_off) and the calculated dissociation constants (K_d) were compared to data obtained from surface plasmon resonance (SPR) measurements. In the present study we identified a good agreement of the determined kinetic constants by the two methods investigated.

Citation Download Citation

Jens Sobek, Stefan Schauer, Hubert Rehrauer, David Fischer, Andrea Patrignani, Stephan Landgraf, and Ralph Schlapbach "Single molecule interactions studied by using a modified DNA sequencer: a comparison with surface plasmon resonance", Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 85960Y (21 February 2013); https://doi.org/10.1117/12.2002953

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available