Imaging pigment chemistry in melanocytic conjunctival lesions with pump-probe microscopy

Jesse W. Wilson; Lejla Vajzovic; Francisco E. Robles; Thomas J. Cummings; Prithvi Mruthyunjaya; Warren S. Warren

doi:10.1117/12.2003137

26 March 2013 Imaging pigment chemistry in melanocytic conjunctival lesions with pump-probe microscopy

Jesse W. Wilson, Lejla Vajzovic, Francisco E. Robles, Thomas J. Cummings, Prithvi Mruthyunjaya, Warren S. Warren

Proceedings Volume 8567, Ophthalmic Technologies XXIII; 856718 (2013) https://doi.org/10.1117/12.2003137
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We extend nonlinear pump-probe microscopy, recently demonstrated to image the microscopic distribution of eumelanin and pheomelanin in unstained skin biopsy sections, to the case of melanocytic conjunctival lesions. The microscopic distribution of pigmentation chemistry serves as a functional indicator of melanocyte activity. In these conjunctival specimens (benign nevi, primary acquired melanoses, and conjunctival melanoma), we have observed pump-probe spectroscopic signatures of eumelanin, pheomelanin, hemoglobin, and surgical ink, in addition to important structural features that differentiate benign from malignant lesions. We will also discuss prospects for an in vivo ‘optical biopsy’ to provide additional information before having to perform invasive procedures.

Citation Download Citation

Jesse W. Wilson, Lejla Vajzovic, Francisco E. Robles, Thomas J. Cummings, Prithvi Mruthyunjaya, and Warren S. Warren "Imaging pigment chemistry in melanocytic conjunctival lesions with pump-probe microscopy", Proc. SPIE 8567, Ophthalmic Technologies XXIII, 856718 (26 March 2013); https://doi.org/10.1117/12.2003137

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KEYWORDS

Melanoma

Biopsy

Microscopy

Chemistry

Principal component analysis

Microscopes

Modulation

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