Multiplexed fluorescence detection for point of care

Li Shen; Mike Ratterman; Tyler Stites; David Klotzkin; Ian Papautsky

doi:10.1117/12.910061

14 February 2012 Multiplexed fluorescence detection for point of care

Li Shen, Mike Ratterman, Tyler Stites, David Klotzkin, Ian Papautsky

Proceedings Volume 8251, Microfluidics, BioMEMS, and Medical Microsystems X; 82510S (2012) https://doi.org/10.1117/12.910061
Event: SPIE MOEMS-MEMS, 2012, San Francisco, California, United States

Abstract

We report on the development of a portable fluorescence detection system. By combining a CMOS sensor and crosspolarization scheme, we achieved multiplexed detection with a single white emission LED excitation. We demonstrated fluorescence detection of Fluorescein and Rhodamine B in PDMS channels and achieved 1μM limit of detection (LOD). Microparticles with green and red fluorescence were detected simultaneously without changing the light sources or filters. We were able to clearly resolve microparticles, even if aggregated. The compact microfluorescence approach offers high spatial and spectral resolution, and is suitable for multiplexed detection in point-of-care applications.

Citation Download Citation

Li Shen, Mike Ratterman, Tyler Stites, David Klotzkin, and Ian Papautsky "Multiplexed fluorescence detection for point of care", Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510S (14 February 2012); https://doi.org/10.1117/12.910061

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