Surface plasmon enhanced-field fluorescence biosensor for point-of-care testing using fluorescent nanoparticles

Kazuyoshi Horii; Toshihito Kimura; Hisashi Ohtsuka; Noriyuki Kasagi; Tomoya Oohara; Tadahiro Matsuno; Masashi Hakamata; Akihiro Komatsu; Tomonari Sendai

doi:10.1117/12.908099

6 February 2012 Surface plasmon enhanced-field fluorescence biosensor for point-of-care testing using fluorescent nanoparticles

Kazuyoshi Horii, Toshihito Kimura, Hisashi Ohtsuka, Noriyuki Kasagi, Tomoya Oohara, Tadahiro Matsuno, Masashi Hakamata, Akihiro Komatsu, Tomonari Sendai

Author Affiliations +

Proceedings Volume 8234, Plasmonics in Biology and Medicine IX; 82340V (2012) https://doi.org/10.1117/12.908099
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

An optical biosensor system using surface-plasmon field-enhanced fluorescence has been developed, which allows high sensitivity and fast measurement available. Intensity of fluorophores in SPFS is highly dependent upon the distance from metal surface. The resonant evanescent electric field excites fluorophores within the penetration area. On the other hand, fluorescence quenching in close proximity to a metal surface interfere with the excitation. We have developed a new technology for fluorescent nanoparticles that could receive the energy from metal surface effectively. This enables technology of detecting strong and stable SPFS signals, as well as homogeneous assay method that allows us to eliminate binding/free separation process for unreacted fluorescent particles. A rate assay method has also been employed, which resolves affect from diffusion-limited access, in order to realize a fast surface immunoreaction in a microchannel. Taking advantage of these two developments, as eliminating an enzyme response process such as CLEIA, our system reaches much faster reaction time of 2 minutes to detect thyroid stimulating hormone (TSH) of canine serum sample at 0.1ng/mL. We believe our system with these new technologies is a powerful tool for in-vitro diagnosis which meets various clinical requirements.

Citation Download Citation

Kazuyoshi Horii, Toshihito Kimura, Hisashi Ohtsuka, Noriyuki Kasagi, Tomoya Oohara, Tadahiro Matsuno, Masashi Hakamata, Akihiro Komatsu, and Tomonari Sendai "Surface plasmon enhanced-field fluorescence biosensor for point-of-care testing using fluorescent nanoparticles", Proc. SPIE 8234, Plasmonics in Biology and Medicine IX, 82340V (6 February 2012); https://doi.org/10.1117/12.908099

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