Fluorescence lifetime-based glucose sensor using NADH

A. von Ketteler; D. Siegberg; D. P. Herten; C. Horn; W. Petrich

doi:10.1117/12.908834

2 February 2012 Fluorescence lifetime-based glucose sensor using NADH

A. von Ketteler, D. Siegberg, D. P. Herten, C. Horn, W. Petrich

Proceedings Volume 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV; 82290P (2012) https://doi.org/10.1117/12.908834
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Fluorescence lifetime-based glucose sensing does not depend on fluctuations of the intensity of the light source, light scattering, or changes in the transmission of optical components. Here we demonstrate the sensing of glucose based on the fluorescence lifetime properties of dihydro nicotinamide adenine dinucleotide (NADH), which is reduced from NAD in the presence of glucose and glucose dehydrogenase. In particular we use the difference in the fluorescence properties of free and protein-bound NADH and calculate an average fluorescence lifetime, which arises from the two short lifetimes τ₁=0.28ns and τ₂=0.60ns (representing free NADH) and the longer lifetime of τ₃=2.9ns (for the protein-bound NADH). While initial results were derived from measurements in aqueous solution, we also demonstrate the suitability of this method for determining the concentration of glucose in blood using test strips. We find that the average fluorescence lifetime changes linearly by a factor of 0.17 per 100mg/_dl change in glucose concentration. As an alternative the ratio between free and protein-bound components R_s/l may also be used for quantification. Rs/l increases by a factor of 0.74 per 100mg/dl change in glucose concentration.

Citation Download Citation

A. von Ketteler, D. Siegberg, D. P. Herten, C. Horn, and W. Petrich "Fluorescence lifetime-based glucose sensor using NADH", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82290P (2 February 2012); https://doi.org/10.1117/12.908834

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