Paper
9 February 2012 Multiwavelength FLIM: new concept for fluorescence diagnosis
Angelika Rück, S. Lorenz, Carmen Hauser, S. Mosch, S. Kalinina
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Abstract
Fluorescence guided tumor resection is very well accepted in the case of bladder cancer and brain tumor, respectively. However, false positive results are one of the major problems, which will make the discrimination between tumor tissue and inflammation difficult. In contrast fluorescence lifetime imaging (FLIM) and especially spectral resolved FLIM (SLIM) can significantly improve the analysis. The fluorescence decay of a fluorophore in many cases does not show a simple monoexponential profile. A very complex situation arises, when more than one compound has to be analyzed. This could be the case when endogenous fluorophores of living cells and tissues have to be discriminated to identify oxidative metabolic changes. Other examples are PDT, when different photosensitizer metabolites are observed simultaneously. In those cases a considerable improvement could be achieved when time-resolved and spectral-resolved techniques are simultaneously incorporated. Within this presentation the principles of spectral and time-resolved fluorescence imaging will be discussed. Successful applications as autofluorescence and 5-ALA induced porphyrin fluorescence will be described in more detail.
© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Angelika Rück, S. Lorenz, Carmen Hauser, S. Mosch, and S. Kalinina "Multiwavelength FLIM: new concept for fluorescence diagnosis", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260G (9 February 2012); https://doi.org/10.1117/12.906620
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Cited by 3 scholarly publications.
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KEYWORDS
Luminescence

Fluorescence lifetime imaging

Content addressable memory

Mode conditioning cables

Tissues

Tumors

Sensors

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