A new optical method for a fast and simple detection of ephedrine

Antonio Varriale; Maria Staiano; Maria Strianese; Vincenzo Marzullo; Giuseppe Ruggiero; Alberto Secchi; Massimiliano Dispenza; Anna Maria Fiorello; Sabato D'Auria

doi:10.1117/12.898045

13 October 2011 A new optical method for a fast and simple detection of ephedrine

Antonio Varriale, Maria Staiano, Maria Strianese, Vincenzo Marzullo, Giuseppe Ruggiero, Alberto Secchi, Massimiliano Dispenza, Anna Maria Fiorello, Sabato D'Auria

Author Affiliations +

Proceedings Volume 8189, Optics and Photonics for Counterterrorism and Crime Fighting VII; Optical Materials in Defence Systems Technology VIII; and Quantum-Physics-based Information Security; 81890M (2011) https://doi.org/10.1117/12.898045
Event: SPIE Security + Defence, 2011, Prague, Czech Republic

Abstract

In this work we describe the synthesis of a new ephedrine derivative with a carbon linker featuring an amino reactive group, and its conjugation to the glutamine binding protein (GlnBP) from E. coli as a carrier protein for the production of polyclonal antibodies in rabbits against ephedrine. Proof-of-principle results that an efficient SPR-based indirect competitive immunoassay for the detection and quantification of ephedrine are presented. The detection limit of this assay was found to be about 33ng/ml.

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Antonio Varriale, Maria Staiano, Maria Strianese, Vincenzo Marzullo, Giuseppe Ruggiero, Alberto Secchi, Massimiliano Dispenza, Anna Maria Fiorello, and Sabato D'Auria "A new optical method for a fast and simple detection of ephedrine", Proc. SPIE 8189, Optics and Photonics for Counterterrorism and Crime Fighting VII; Optical Materials in Defence Systems Technology VIII; and Quantum-Physics-based Information Security, 81890M (13 October 2011); https://doi.org/10.1117/12.898045

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