Two-dimensional phase imaging based on closed-loop feedback control

Eriko Watanabe; Miki Toshima; Chiemi Fujikawa

doi:10.1117/12.893503

22 September 2011 Two-dimensional phase imaging based on closed-loop feedback control

Eriko Watanabe, Miki Toshima, Chiemi Fujikawa

Author Affiliations +

Proceedings Volume 8134, Optics and Photonics for Information Processing V; 81340G (2011) https://doi.org/10.1117/12.893503
Event: SPIE Optical Engineering + Applications, 2011, San Diego, California, United States

Abstract

Quantitative phase microscopy with a closed-loop feedback control is described. The system is based on a Mach-Zehnder interferometer using a phase-locking technique and measures the change in optical path length while the sample is scanned across the optical axis. The important feature of this system is no need for any unwrapping process, which allows us to measure the sample with highly precise around 2π radian. The spatial resolution is estimated to be less than 1μm, which is limited by the Rayleigh criterion and the sample thickness. The path length resolution is estimated to be less than 1nm, which corresponds to the change in refractive index less than 10^-3 for the sample of 10μm thick. Precise measurements of quantitative phase images by a volume phase holographic grating or a multi-level zone plate array validate the principle and show the accuracy of the methods. The samples depending on wavelength with the pigment such as red blood cell and diatom cells are measured by different wavelength. We quantify its accuracy by measuring calibrated test samples. The technique is applicable to examination of limitation of passage culture, biological cell imaging for diagnosis.

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Eriko Watanabe, Miki Toshima, and Chiemi Fujikawa "Two-dimensional phase imaging based on closed-loop feedback control", Proc. SPIE 8134, Optics and Photonics for Information Processing V, 81340G (22 September 2011); https://doi.org/10.1117/12.893503

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KEYWORDS

Refractive index

Phase measurement

Feedback control

Blood

Volume holography

Holography

Ferroelectric materials

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