Plasma membrane microorganization of LR73 multidrug-resistant cells revealed by FCS

Pascale Winckler; Rodolphe Jaffiol; Aurélie Cailler; Hamid Morjani; Pierre Jeannesson; Régis Deturche

doi:10.1117/12.874496

23 February 2011 Plasma membrane microorganization of LR73 multidrug-resistant cells revealed by FCS

Pascale Winckler, Rodolphe Jaffiol, Aurélie Cailler, Hamid Morjani, Pierre Jeannesson, Régis Deturche

Proceedings Volume 7905, Single Molecule Spectroscopy and Imaging IV; 79050I (2011) https://doi.org/10.1117/12.874496
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Tumoral cells could present a multidrug resistance (MDR) to chemotherapeutic treatments. This drug resistance would be associated to biomechanisms occurring at the plasma membrane level, involving modification of membrane fluidity, drug permeability, presence of microdomains (rafts, caveolae...), and membrane proteins overexpression such as Pglycoprotein. Fluorescence correlation spectroscopy (FCS) is the relevant method to investigate locally the fluidity of biological membranes through the lateral diffusion of a fluorescent membrane probe. Thus, we use FCS to monitor the plasma membrane local organization of LR73 carcinoma cells and three derived multidrug-resistant cancer cells lines. Measurements were conducted at the single cell level, which enabled us to get a detailed overview of the plasma membrane microviscosity distribution of each cell line studied. Moreover, we propose 2D diffusion simulation based on a Monte Carlo model to investigate the membrane organisation in terms of microdomains. This simulation allows us to relate the differences in the fluidity distributions with microorganization changes in plasma membrane of MDR cells.

Citation Download Citation

Pascale Winckler, Rodolphe Jaffiol, Aurélie Cailler, Hamid Morjani, Pierre Jeannesson, and Régis Deturche "Plasma membrane microorganization of LR73 multidrug-resistant cells revealed by FCS", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050I (23 February 2011); https://doi.org/10.1117/12.874496

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