Quadriwave lateral shearing interferometry for quantitative phase microscopy: coupling phase imaging and fluorescence imaging

Pierre Bon; Julien Savatier; Benoit Wattellier; Didier Marguet; Serge Monneret

doi:10.1117/12.873177

22 February 2011 Quadriwave lateral shearing interferometry for quantitative phase microscopy: coupling phase imaging and fluorescence imaging

Pierre Bon, Julien Savatier, Benoit Wattellier, Didier Marguet, Serge Monneret

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Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 79040P (2011) https://doi.org/10.1117/12.873177
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Phase imaging with a high-resolution wavefront sensor is a useful setup for biological imaging. Our setup is based on a quadriwave lateral shearing interferometer mounted on a commercial non-modified transmission white-light microscope. That allows us to make simultaneous measurement in both quantitative transmission phase and fluorescence imaging. We propose here to study co-localization between phase and fluorescence on african green monkey kidney COS7 cells. Phase permits an enhanced visualization of the whole cell and intracellular components while the fluorescence allows a complete identification of each component. Post treatments on phase-shift images are proposed and become very interesting for enhanced visualization of small details such as vesicles or mitochondrias.

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Pierre Bon, Julien Savatier, Benoit Wattellier, Didier Marguet, and Serge Monneret "Quadriwave lateral shearing interferometry for quantitative phase microscopy: coupling phase imaging and fluorescence imaging", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040P (22 February 2011); https://doi.org/10.1117/12.873177

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KEYWORDS

Luminescence

Phase measurement

Image filtering

Optical filters

Cameras

Microscopes

Phase imaging

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