Development of a stigmatic imaging mass spectrometer using laser desorption/ionization

Kunio Awazu; Hisanao Hazama; Hirofumi Nagao; Hidetoshi Yoshimura; Jun Aoki; Kenichi Fujii; Katsuyoshi Masuda; Toshio Tashima; Michisato Toyoda; Yasuhide Naito

doi:10.1117/12.876005

23 February 2011 Development of a stigmatic imaging mass spectrometer using laser desorption/ionization

Kunio Awazu, Hisanao Hazama, Hirofumi Nagao, Hidetoshi Yoshimura, Jun Aoki, Kenichi Fujii, Katsuyoshi Masuda, Toshio Tashima, Michisato Toyoda, Yasuhide Naito

Author Affiliations +

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 790225 (2011) https://doi.org/10.1117/12.876005
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Imaging mass spectrometry based on matrix-assisted laser desorption/ionization gives distributions of multiple biomolecules and identification of analytes in a tissue section. We are developing a stigmatic imaging mass spectrometer using a multi-turn time-of-flight mass spectrometer, MULTUM-IMG. A mesh pattern image was obtained with spatial resolution of 3 μm. Separation of stigmatic ion images according to the mass-to-charge ratio was also successfully demonstrated with the micro-dot pattern made with crystal violet and methylene blue. An ion image of a micro-dot pattern sample was observed after ten cycles in the multi-turn circuit of MULTUM-IMG, and the pattern of the sample was maintained. Then, stigmatic ion images of the ions of crystal violet and methylene blue produced from the stained section of a brain were observed. An ion image of whole part of a hippocampus (3.25 mm × 1.5 mm) in the brain section can be obtained within 13 minutes.

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Kunio Awazu, Hisanao Hazama, Hirofumi Nagao, Hidetoshi Yoshimura, Jun Aoki, Kenichi Fujii, Katsuyoshi Masuda, Toshio Tashima, Michisato Toyoda, and Yasuhide Naito "Development of a stigmatic imaging mass spectrometer using laser desorption/ionization", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790225 (23 February 2011); https://doi.org/10.1117/12.876005

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KEYWORDS

Ions

Spectroscopy

Electrodes

Brain

Crystals

Spatial resolution

Tissues

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