Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber

Mir Farrokh Salek; Mark D. Pagel; Arthur F. Gmitro

doi:10.1117/12.876529

3 March 2011 Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber

Mir Farrokh Salek, Mark D. Pagel, Arthur F. Gmitro

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 79020G (2011) https://doi.org/10.1117/12.876529
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Optical imaging and MRI have both been used extensively to study tumor microenvironment. The two imaging modalities are complementary and can be used to cross-validate one another for specific measurements. We have developed a modular platform that is capable of doing optical microscopy inside an MRI instrument. To do this, an optical relay system transfers the image to outside of the MR bore to a commercial grade CCD camera. This enables simultaneous optical and MR imaging of the same tissue and thus creates the ideal situation for comparative or complementary studies using both modalities. Initial experiments have been done using GFP labeled prostate cancer cells implanted in mouse dorsal skin fold window chamber. Vascular hemodynamics and vascular permeability were studied using our imaging system. Towards this goal, we developed a dual MR-Optical contrast agent by labeling BSA with both Gd-DTPA and Alexa Fluor. Overall system design and results of these preliminary vascular studies are presented.

Citation Download Citation

Mir Farrokh Salek, Mark D. Pagel, and Arthur F. Gmitro "Simultaneous dual modality optical and MR imaging of mouse dorsal skin-fold window chamber", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020G (3 March 2011); https://doi.org/10.1117/12.876529

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