Label free detection of DNA hybridization by refractive index tapered fiber biosensor

M. I. Zibaii; H. Latifi; E. Ghanati; M. Gholami; S. M. Hosseini

doi:10.1117/12.853974

18 May 2010 Label free detection of DNA hybridization by refractive index tapered fiber biosensor

M. I. Zibaii, H. Latifi, E. Ghanati, M. Gholami, S. M. Hosseini

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 77151Z (2010) https://doi.org/10.1117/12.853974
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

We demonstrate a simple refractive index sensor (RI) sensing system based on a biconical tapered optical fiber (BTOF), which is fabricated by heat pulling method, utilizing a CO₂ laser. In this work we explore the application of these sensors for the detection of label free single stranded DNA (ssDNA) in real time. During the experiment, the target ssDNA did not need to be labeled with a fluorescent tag, which is expensive and complicated. The change in output optical transmission of the tapered fiber was recorded for Poly-L-Lysine (PLL) coating, ssDNA probe immobilization and hybridization. The result indicated that due to the hybridization with the complementary target ssDNA on the tapered surface, the RI of surrounding medium changes which leads to changes in the characteristics of the tapered region and change in the output power of the sensor.

Citation Download Citation

M. I. Zibaii, H. Latifi, E. Ghanati, M. Gholami, and S. M. Hosseini "Label free detection of DNA hybridization by refractive index tapered fiber biosensor", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151Z (18 May 2010); https://doi.org/10.1117/12.853974

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