Biomolecular detection using a metal semiconductor field effect transistor

Elias Estephan; Marie-Belle Saab; Petre Buzatu; Roger Aulombard; Frédéric J. G. Cuisinier; Csilla Gergely; Thierry Cloitre

doi:10.1117/12.853536

17 May 2010 Biomolecular detection using a metal semiconductor field effect transistor

Elias Estephan, Marie-Belle Saab, Petre Buzatu, Roger Aulombard, Frédéric J. G. Cuisinier, Csilla Gergely, Thierry Cloitre

Author Affiliations +

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 77151V (2010) https://doi.org/10.1117/12.853536
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

In this work, our attention was drawn towards developing affinity-based electrical biosensors, using a MESFET (Metal Semiconductor Field Effect Transistor). Semiconductor (SC) surfaces must be prepared before the incubations with biomolecules. The peptides route was adapted to exceed and bypass the limits revealed by other types of surface modification due to the unwanted unspecific interactions. As these peptides reveal specific recognition of materials, then controlled functionalization can be achieved. Peptides were produced by phage display technology using a library of M13 bacteriophage. After several rounds of bio-panning, the phages presenting affinities for GaAs SC were isolated; the DNA of these specific phages were sequenced, and the peptide with the highest affinity was synthesized and biotinylated. To explore the possibility of electrical detection, the MESFET fabricated with the GaAs SC were used to detect the streptavidin via the biotinylated peptide in the presence of the bovine Serum Albumin. After each surface modification step, the IDS (current between the drain and the source) of the transistor was measured and a decrease in the intensity was detected. Furthermore, fluorescent microscopy was used in order to prove the specificity of this peptide and the specific localisation of biomolecules. In conclusion, the feasibility of producing an electrical biosensor using a MESFET has been demonstrated. Controlled placement, specific localization and detection of biomolecules on a MESFET transistor were achieved without covering the drain and the source. This method of functionalization and detection can be of great utility for biosensing application opening a new way for developing bioFETs (Biomolecular Field-Effect Transistor).

Citation Download Citation

Elias Estephan, Marie-Belle Saab, Petre Buzatu, Roger Aulombard, Frédéric J. G. Cuisinier, Csilla Gergely, and Thierry Cloitre "Biomolecular detection using a metal semiconductor field effect transistor", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151V (17 May 2010); https://doi.org/10.1117/12.853536

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Field effect transistors

Gallium arsenide

Semiconductors

Transistors

Metals

Microscopy

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years