A microfluidic platform for chip-based DNA detection using SERS and silver colloids

K. K. Strelau; K. Weber; R. Möller; W. Fritzsche; J. Popp

doi:10.1117/12.854268

28 April 2010 A microfluidic platform for chip-based DNA detection using SERS and silver colloids

K. K. Strelau, K. Weber, R. Möller, W. Fritzsche, J. Popp

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 771514 (2010) https://doi.org/10.1117/12.854268
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

Within this contribution, we demonstrate a combination of microarrays, microfluidics and SERS to enable a sequence specific detection of DNA. In this combination, the microarray allows for the immobilisation of DNA sequences as well as the removal of unbound DNA, microfluidics permit the automation of the process and SERS provides a highly sensitive detection by means of an interaction between an analyte molecule and the enhanced electromagnetic field in the proximity of metallic nanostructured surfaces such as spherical nanoparticles. With this setup, we are able to distinguish between different complementary and non-complementary target sequences in one sample solution.

Citation Download Citation

K. K. Strelau, K. Weber, R. Möller, W. Fritzsche, and J. Popp "A microfluidic platform for chip-based DNA detection using SERS and silver colloids", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771514 (28 April 2010); https://doi.org/10.1117/12.854268

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