Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles

Etienne Shaffer; Christian Depeursinge

doi:10.1117/12.854289

14 May 2010 Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 771505 (2010) https://doi.org/10.1117/12.854289
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index across a sample (e.g. cell or neuron). In short,DHM added a new dimension to optical imaging,whic h explains why it is such an excellent instrument for metrological,but also for biological applications. We believe that DHM is,b y nature,ideally suited for nonlinear microscopy. In this work,w e review the advantages of DHM for nonlinear microscopy and present its application to determination of the axial position of nonlinear nanoparticles capable of second harmonic generation.

Citation Download Citation

Etienne Shaffer and Christian Depeursinge "Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771505 (14 May 2010); https://doi.org/10.1117/12.854289

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available