Transferrin protein nanospheres: a nanoplatform for receptor-mediated cancer cell labeling and gene delivery

Michael A. McDonald; Tighe A. Spurlin; Alessandro Tona; John T. Elliott; Michael Halter; Anne L. Plant

doi:10.1117/12.842757

16 February 2010 Transferrin protein nanospheres: a nanoplatform for receptor-mediated cancer cell labeling and gene delivery

Michael A. McDonald, Tighe A. Spurlin, Alessandro Tona, John T. Elliott, Michael Halter, Anne L. Plant

Author Affiliations +

Proceedings Volume 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II; 75760J (2010) https://doi.org/10.1117/12.842757
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

This paper presents preliminary results on the use of transferrin protein nanospheres (TfpNS) for targeting cancer cells in vitro. Protein nanospheres represent an easily prepared and modifiable nanoplatform for receptor-specific targeting, molecular imaging and gene delivery. Rhodamine B isothiocyanate conjugated TfpNS (RBITC-TfpNS) show significantly enhanced uptake in vitro in SK-MEL-28 human malignant melanoma cells known to overexpress transferrin receptors compared to controls. RBITCTfpNS labeling of the cancer cells is due to transferrin receptor-mediated uptake, as demonstrated by competitive inhibition with native transferrin. Initial fluorescence microscopy studies indicate GFP plasmid can be transfected into melanoma cells via GFP plasmid encapsulated by TfpNS.

Citation Download Citation

Michael A. McDonald, Tighe A. Spurlin, Alessandro Tona, John T. Elliott, Michael Halter, and Anne L. Plant "Transferrin protein nanospheres: a nanoplatform for receptor-mediated cancer cell labeling and gene delivery", Proc. SPIE 7576, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications II, 75760J (16 February 2010); https://doi.org/10.1117/12.842757

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