Optical size determination of quantum dots using FRET with terbium complexes as donors

D. Geißler; H.-G. Löhmannsröben; L. J. Charbonnière; R. F. Ziessel; N. G. Butlin; I. L. Medintz; H. Mattoussi; N. Hildebrandt

doi:10.1117/12.842750

17 February 2010 Optical size determination of quantum dots using FRET with terbium complexes as donors

D. Geißler, H.-G. Löhmannsröben, L. J. Charbonnière, R. F. Ziessel, N. G. Butlin, I. L. Medintz, H. Mattoussi, N. Hildebrandt

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Proceedings Volume 7575, Colloidal Quantum Dots for Biomedical Applications V; 75750E (2010) https://doi.org/10.1117/12.842750
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We present the use of luminescent terbium complexes (LTCs) as FRET donors and luminescent semiconductor quantum dots (QDs) as FRET acceptors for spectroscopic ruler measurements. The LTCs were labeled to polyhistidine-appended peptides which self-assembled onto three different QDs allowing optical multiplexed measurements. Forster distances were in the range of 60-75 Å and FRET efficiencies of up to 97 % were realized. Time-resolved analysis allowed the determination of donor-acceptor separation distances. The results suggests the efficient use of our LTC-to-QD FRET systems for multiplexed optical size determination, molecular ruler measurements and multi-parameter diagnostics.

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D. Geißler, H.-G. Löhmannsröben, L. J. Charbonnière, R. F. Ziessel, N. G. Butlin, I. L. Medintz, H. Mattoussi, and N. Hildebrandt "Optical size determination of quantum dots using FRET with terbium complexes as donors", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 75750E (17 February 2010); https://doi.org/10.1117/12.842750

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