The spectroscopic manifestation of the interaction of some cyanine dyes with BSA and DNA

Eldar N. Kurtaliev; Negmat Nizomov; Sherzod I. Rahimov; Gayrat Khodjayev; Dilorom P. Khakimova

doi:10.1117/12.840722

25 February 2010 The spectroscopic manifestation of the interaction of some cyanine dyes with BSA and DNA

Eldar N. Kurtaliev, Negmat Nizomov, Sherzod I. Rahimov, Gayrat Khodjayev, Dilorom P. Khakimova

Proceedings Volume 7571, Single Molecule Spectroscopy and Imaging III; 757116 (2010) https://doi.org/10.1117/12.840722
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The spectral-luminescent characteristics of cyanine dye Cyan 40 and thiazole orange at interaction with biomacromolecules bovine serum albumin and deoxyribonucleic acid was studied. It is shown that presence of biomacromolecules in solution of the studied species leads to the change of spectral-luminescent characteristics of the dyes Cyan 40 and TO. The observed phenomena in absorption spectrum and fluorescence of the studied dyes is explained by complex formation between dye and biological macromolecules. The binding parameters: a binding constant (K) and quantity of the binding sites (N) the studied dyes with biomacromolecules are determined.

Citation Download Citation

Eldar N. Kurtaliev, Negmat Nizomov, Sherzod I. Rahimov, Gayrat Khodjayev, and Dilorom P. Khakimova "The spectroscopic manifestation of the interaction of some cyanine dyes with BSA and DNA", Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 757116 (25 February 2010); https://doi.org/10.1117/12.840722

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