Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

M. Fatih Toy; Christophe Pache; Jérôme Parent; Jonas Kühn; Marcel Egli; Christian Depeursinge

doi:10.1117/12.842779

24 February 2010 Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

M. Fatih Toy, Christophe Pache, Jérôme Parent, Jonas Kühn, Marcel Egli, Christian Depeursinge

Author Affiliations +

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700A (2010) https://doi.org/10.1117/12.842779
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic Microscopy (DHM) and widefield epifluorescence microscopy modes in a time sequential basis. DHM provides information on real time cellular morphology. EGFP transfected actin filaments in mouse myoblast cells function as the reporter for the fluorescence microscopy mode. Experimental setup is fixed in the RPM to observe microgravity induced dynamic changes in live cells. Initial results revealed two different modifications. Disorganized structures become visible in the formed lamellipodias, and proteins accumulate in the perinuclear region.

Citation Download Citation

M. Fatih Toy, Christophe Pache, Jérôme Parent, Jonas Kühn, Marcel Egli, and Christian Depeursinge "Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700A (24 February 2010); https://doi.org/10.1117/12.842779

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