Closed loop adaptive optics for microscopy without a wavefront sensor

Peter Kner; Lukman Winoto; David A. Agard; John W. Sedat

doi:10.1117/12.840943

24 February 2010 Closed loop adaptive optics for microscopy without a wavefront sensor

Peter Kner, Lukman Winoto, David A. Agard, John W. Sedat

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 757006 (2010) https://doi.org/10.1117/12.840943
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A three-dimensional wide-field image of a small fluorescent bead contains more than enough information to accurately calculate the wavefront in the microscope objective back pupil plane using the phase retrieval technique. The phase-retrieved wavefront can then be used to set a deformable mirror to correct the point-spread function (PSF) of the microscope without the use of a wavefront sensor. This technique will be useful for aligning the deformable mirror in a widefield microscope with adaptive optics and could potentially be used to correct aberrations in samples where small fluorescent beads or other point sources are used as reference beacons. Another advantage is the high resolution of the retrieved wavefont as compared with current Shack-Hartmann wavefront sensors. Here we demonstrate effective correction of the PSF in 3 iterations. Starting from a severely aberrated system, we achieve a Strehl ratio of 0.78 and a greater than 10-fold increase in maximum intensity.

Citation Download Citation

Peter Kner, Lukman Winoto, David A. Agard, and John W. Sedat "Closed loop adaptive optics for microscopy without a wavefront sensor", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 757006 (24 February 2010); https://doi.org/10.1117/12.840943

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