Object Identification And Measurement From Images With Access To The Database To Select Specific Subpopulations Of Special Interest

H. G. Rutherford; F. E. Taub; B. Williams

doi:10.1117/12.940046

3 June 1987 Object Identification And Measurement From Images With Access To The Database To Select Specific Subpopulations Of Special Interest

H. G. Rutherford, F. E. Taub, B. Williams

Author Affiliations +

Proceedings Volume 0757, Methods of Handling and Processing Imagery; (1987) https://doi.org/10.1117/12.940046
Event: OE LASE'87 and EO Imaging Symposium, 1987, Los Angeles, CA, United States

Abstract

An automated vision image analysis system to analyze images resulting from nucleic acid hybridizations is presented. Real-time image acquisition from autoradiographs is accomplished with a sensitivity to optical density to the degree that any spot visible by eye can be quantified by digital methods and enhanced by image processing techniques for observation. The system provides a tool for quantification of differences in gene expression, rapid processing of thousands of pairs of genes, storage in a database, search for small differences, large differences, and infrequently occuring genes. Background correction, normalization and pseudocolor "mapping" facilitate comparison and quantification directly from the CRT display. The image pairs, a percent change image and a difference image are presented in a quad display with coordinated spot density and change measurement simultaneously for each of the four images. The system provides a tool for analysis of altered gene expression in response to environmental stress, such as heat-shock and salt water in plant growth; hormone regulation, such as before and after hormone administration in animals; development; and carcinogenesis. The use of this system to study nucleic acid hybridization is contrasted with the study of 2-D protein electrophoresis gels for the determination of changes in gene expression and identification of corresponding proteins of significance.

Citation Download Citation

H. G. Rutherford, F. E. Taub, and B. Williams "Object Identification And Measurement From Images With Access To The Database To Select Specific Subpopulations Of Special Interest", Proc. SPIE 0757, Methods of Handling and Processing Imagery, (3 June 1987); https://doi.org/10.1117/12.940046

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available