Multiplex coherent anti-Stokes Raman scattering flow cytometry for real-time classification of particles in a microfluidic channel

Charles H. Camp Jr.; Siva Yegnanarayanan; Ali A. Eftekhar; Hamsa Sridhar; Ali Adibi

doi:10.1117/12.843011

26 February 2010 Multiplex coherent anti-Stokes Raman scattering flow cytometry for real-time classification of particles in a microfluidic channel

Charles H. Camp Jr., Siva Yegnanarayanan, Ali A. Eftekhar, Hamsa Sridhar, Ali Adibi

Author Affiliations +

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75692F (2010) https://doi.org/10.1117/12.843011
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Flow cytometry is an ever-advancing, multivariate analysis tool obtaining morphological and phenotype information from sample populations. In traditional flow cytometers, obtaining molecular information requires the use of endogenous fluorophores, which are limited by spectral overlap, nonspecific binding, available conjugation chemistries, and cellular toxicity. In this work, however, we apply multiplex coherent anti-Stokes Raman scattering (MCARS), a nonlinear optical method that probes the Raman energies within a molecule, to flow cytometry in order to demonstrate label-free, molecularly sensitive particle differentiation. To demonstrate this, we correctly differentiate 5 μm polystyrene (PS) and poly(methyl methacrylate) (PMMA), which (linearly) optically appear similar, but vary significantly in their MCARS spectrum.

Citation Download Citation

Charles H. Camp Jr., Siva Yegnanarayanan, Ali A. Eftekhar, Hamsa Sridhar, and Ali Adibi "Multiplex coherent anti-Stokes Raman scattering flow cytometry for real-time classification of particles in a microfluidic channel", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692F (26 February 2010); https://doi.org/10.1117/12.843011

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