Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors

Keith M. Stantz; Ning Cao; Bo Liu; Minsong Cao; Helen Chin-Sinex; Marc Mendonca; Jian Jian Li

doi:10.1117/12.842238

23 February 2010 Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors

Keith M. Stantz, Ning Cao, Bo Liu, Minsong Cao, Helen Chin-Sinex, Marc Mendonca, Jian Jian Li

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75641J (2010) https://doi.org/10.1117/12.842238
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Purpose: The purpose of this study is to monitor in vivo the IR dose dependent response of NF-κB and tumor hemodynamics as a function of time. Material and Methods: An MDA-231 breast cancer cell line was stably transfected with a firefly luciferase gene within the NF-kappaB promoter. Tumors on the right flank irradiated with a single fractionated dose of 5Gy or 10Gy. Over two weeks, photoacoustic spectroscopy (PCT-S), bioluminescence imaging (BLI), and dynamic contrast enhanced CT (DCE-CT) was used to monitor hemoglobin status, NF-kappaB expression, and physiology, respectively. Results: From the BLI, an increase in NF-kappaB expression was observed in both the right (irradiation) and left (nonirradiated) tumors, which peaked at 8-12 hours, returned to basal levels after 24 hours, and increased a second time from 3 to 7 days. This data identifies both a radiation-induced bystander effect and a bimodal longitudinal response associated with NF-κB-controlled luciferase promoter. The physiological results from DCE-CT measured an increase in perfusion (26%) two days after radiation and both a decrease in perfusion and an increase in fp by week 1 (10Gy cohort). PCT-S measured increased levels of oxygen saturation two days post IR, which did not change after 1 week. Initially, NF-κB would modify hemodynamics to increase oxygen delivery after IR insult. The secondary response appears to modulate tumor angiogenesis. Conclusions: A bimodal response to radiation was detected with NF-kappaB-controlled luciferase reporter with a concomitant hemodynamic response associated with tumor hypoxia. Experiments are being performed to increase statistics.

Citation Download Citation

Keith M. Stantz, Ning Cao, Bo Liu, Minsong Cao, Helen Chin-Sinex, Marc Mendonca, and Jian Jian Li "Effects of radiation on tumor hemodynamics and NF-kappaB in breast tumors", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75641J (23 February 2010); https://doi.org/10.1117/12.842238

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available