Differentiation of normal and cancerous lung tissues by multiphoton imaging

Chun-Chin Wang; Feng-Chieh Li; Ruei-Jr Wu; Vladimir A. Hovhannisyan; Wei-Chou Lin; Sung-Jan Lin; Peter T. C. So; Chen-Yuan Dong

doi:10.1117/12.843173

11 February 2010 Differentiation of normal and cancerous lung tissues by multiphoton imaging

Chun-Chin Wang, Feng-Chieh Li, Ruei-Jr Wu, Vladimir A. Hovhannisyan, Wei-Chou Lin, Sung-Jan Lin, Peter T. C. So, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 7561, Optical Biopsy VII; 756112 (2010) https://doi.org/10.1117/12.843173
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In this work, we utilized multiphoton microscopy for the label-free diagnosis of non-cancerous, lung adenocarcinoma (LAC), and lung squamous cell carcinoma (SCC) tissues from human. Our results show that the combination of second harmonic generation (SHG) and multiphoton excited autofluorescence (MAF) signals may be used to acquire morphological and quantitative information in discriminating cancerous from non-cancerous lung tissues. Specifically, non-cancerous lung tissues are largely fibrotic in structure while cancerous specimens are composed primarily of tumor masses. Quantitative ratiometric analysis using MAF to SHG index (MAFSI or SAAID) shows that the average MAFSI for noncancerous and LAC lung tissue pairs are 0.55 ±0.23 and 0.87±0.15 respectively. In comparison, the MAFSIs for the noncancerous and SCC tissue pairs are 0.50±0.12 and 0.72±0.13 respectively. Intrinsic fluorescence ratio (FAD/NADH) of SCC and non-cancerous tissues are 0.40±0.05 and 0.53±0.05 respectively, the redox ratio of SCC diminishes significantly, indicating that increased cellular metabolic activity. Our study shows that nonlinear optical microscopy can assist in differentiating and diagnosing pulmonary cancer from non-cancerous tissues. With additional development, multiphoton microscopy may be used for the clinical diagnosis of lung cancers.

Citation Download Citation

Chun-Chin Wang, Feng-Chieh Li, Ruei-Jr Wu, Vladimir A. Hovhannisyan, Wei-Chou Lin, Sung-Jan Lin, Peter T. C. So, and Chen-Yuan Dong "Differentiation of normal and cancerous lung tissues by multiphoton imaging", Proc. SPIE 7561, Optical Biopsy VII, 756112 (11 February 2010); https://doi.org/10.1117/12.843173

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