In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats

Akihiro Hirao; Shunichi Sato; Mitsuhiro Terakawa; Daizoh Saitoh; Nariyoshi Shinomiya; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.841495

4 March 2010 In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats

Akihiro Hirao, Shunichi Sato, Mitsuhiro Terakawa, Daizoh Saitoh, Nariyoshi Shinomiya, Hiroshi Ashida, Minoru Obara

Proceedings Volume 7551, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX; 75510V (2010) https://doi.org/10.1117/12.841495
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Control of infection in wounds is critically important to avoid transition to sepsis; however, recent rise of drug-resistant bacteria makes it difficult. Thus, antimicrobial photodynamic therapy (APDT) has recently received considerable attention. In this study, we examined methylene blue (MB)-mediated photodynamic inactivation of Psuedomonas aeruginosa in rat burned skin. Two days after infection, the wound surface was contacted with a MB solution at different concentrations, and thereafter the wound was irradiated with cw 665-nm light at a constant power density of 250 mW/cm² for different time durations. We obtained a two orders of magnitude decrease in the number of bacteria by PDT with a 2-h contact of 0.5-mM MB solution and a illumination of 480 J/cm², demonstrating the efficacy of PDT against infection with Ps. aeruginosa in burns.

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Akihiro Hirao, Shunichi Sato, Mitsuhiro Terakawa, Daizoh Saitoh, Nariyoshi Shinomiya, Hiroshi Ashida, and Minoru Obara "In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats", Proc. SPIE 7551, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIX, 75510V (4 March 2010); https://doi.org/10.1117/12.841495

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KEYWORDS

Photodynamic therapy

Bacteria

Skin

Picosecond phenomena

Tissues

In vivo imaging

Tissue optics

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