Bypassing the blood-brain barrier: delivery of therapeutic agents by macrophages

Henry Hirschberg; Seung-Kuk Baek; Young Jik Kwon; Chung-Ho Sun; Steen J. Madsen

doi:10.1117/12.846958

2 March 2010 Bypassing the blood-brain barrier: delivery of therapeutic agents by macrophages

Henry Hirschberg, Seung-Kuk Baek, Young Jik Kwon, Chung-Ho Sun, Steen J. Madsen

Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75483Z (2010) https://doi.org/10.1117/12.846958
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Introduction: Failure to eradicate infiltrating glioma cells using conventional treatment regimens results in tumor recurrence and is responsible for the dismal prognosis of patients with glioblastoma multiforme (GBM). This is due to the fact that these migratory cells are protected by the blood-brain barrier (BBB) and the blood brain tumor barrier (BBTB) which prevents the delivery of most anti-cancer agents. We have evaluated the ability of monocytes/macrophages (Mo/Ma) to cross the BBB in rats. This will permit access of anti-cancer agents such as nanoparticles to effectively target the infiltrating tumor cells, and potentially improve the treatment effectiveness for malignant gliomas. Materials and Methods: The infiltration of Mo/Ma into brain tumor spheroids in vitro was determined using fluorescent stained Mo/Ma. Tumors were also established in the brains of inbred rats and ALA-PDT was given 18 days following tumor induction. The degredation of the BBTB and quantification of the number of infiltrating Mo/Ma was examined on histological sections from removed brains. Results & Conclusion: PDT was highly effective in locally opening the BBTB and inducing macrophage migration into the irradiated portions of brain tumors.

Citation Download Citation

Henry Hirschberg, Seung-Kuk Baek, Young Jik Kwon, Chung-Ho Sun, and Steen J. Madsen "Bypassing the blood-brain barrier: delivery of therapeutic agents by macrophages", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75483Z (2 March 2010); https://doi.org/10.1117/12.846958

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