Subsurface imaging and cell refractometry using quantitative phase/ shear-force feedback microscopy

Kert Edward; Faramarz Farahi

doi:10.1117/12.835147

5 October 2009 Subsurface imaging and cell refractometry using quantitative phase/ shear-force feedback microscopy

Kert Edward, Faramarz Farahi

Proceedings Volume 7503, 20th International Conference on Optical Fibre Sensors; 75031M (2009) https://doi.org/10.1117/12.835147
Event: 20th International Conference on Optical Fibre Sensors, 2009, Edinburgh, United Kingdom

Abstract

Over the last few years, several novel quantitative phase imaging techniques have been developed for the study of biological cells. However, many of these techniques are encumbered by inherent limitations including 2π phase ambiguities and diffraction limited spatial resolution. In addition, subsurface information in the phase data is not exploited. We hereby present a novel quantitative phase imaging system without 2 π ambiguities, which also allows for subsurface imaging and cell refractometry studies. This is accomplished by utilizing simultaneously obtained shear-force topography information. We will demonstrate how the quantitative phase and topography data can be used for subsurface and cell refractometry analysis and will present results for a fabricated structure and a malaria infected red blood cell.

Citation Download Citation

Kert Edward and Faramarz Farahi "Subsurface imaging and cell refractometry using quantitative phase/ shear-force feedback microscopy", Proc. SPIE 7503, 20th International Conference on Optical Fibre Sensors, 75031M (5 October 2009); https://doi.org/10.1117/12.835147

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